Mt. Sinai School of Medicine
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Extinction Training During the Reconsolidation Window Prevents Recovery of Fear
Daniela Schiller 1, Candace M. Raio 2, Elizabeth A. Phelps 3
1Departments of Psychiatry and Neuroscience, and Friedman Brain Institute, Mt. Sinai School of Medicine, 2Department of Psychology, New York University , 3Department of Psychology and Center for Neural Science, New York University
Conditioned fear can be diminished through an inhibitory process called extinction, but can resurface under conditions such as the passage of time or exposure to stress. Our protocol presents a novel way of preventing fear recovery by introducing extinction during the reconsolidation window (the re-storage phase of a reactivated memory).
Neuroscience
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
Bjoern Ch. Ludwar 1, Colin G. Evans 1,2, Elizabeth C. Cropper 1
1Fishberg Department of Neuroscience and Friedman Brain Institute, Mt. Sinai School of Medicine, 2Phase Five Communications Inc.
We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.
