Va San Diego Healthcare System
2 ARTICLES PUBLISHED IN JoVE
Medicine
Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope
Joanna L. Thomas 1,2, Justin Dumouchel 2, Jinghong Li 2, Jenna Magat 2, Dana Balitzer 3, Timothy D. Bigby 1,2
1Medical and Research Services, VA San Diego Healthcare System, 2Department of Medicine, University of California, San Diego, 3School of Medicine, University of California, San Diego
We have developed a simple, reliable, and relatively inexpensive method for endotracheal intubation in mice via direct laryngoscopy using an otoscope with a 2.0 mm speculum. This technique is atraumatic and can be used for repeated measurements in chronic experiments. We find it superior to tracheostomy or previously reported nonsurgical techniques.
Neuroscience
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
Xiaobei Zhao 1, Yue Zhou 2, April M. Weissmiller 1, Matthew L. Pearn 3,4, William C. Mobley 1, Chengbiao Wu 1
1Department of Neurosciences, University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 3Department of Anesthesiology, University of California, San Diego, 4VA San Diego Healthcare System
Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.
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