Université Paris-Est

3 ARTICLES PUBLISHED IN JoVE

Medicine

Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis

Mathieu Manassero^1,2, Adeline Decambron^1,2, Bui Truong Huu Thong¹, Véronique Viateau^1,2, Morad Bensidhoum¹, Hervé Petite¹

¹Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires (B2OA - UMR CNRS 7052), Université Paris Diderot, ²Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est

Although mouse models are invaluable tools for bone tissue engineering, models of long bone defects are sparse. This need motivated development of the present protocol which uses a locking plate with four screws and a dedicated jig to perform and stabilize a reproducible, femoral, critical-size defect with low morbidity.

Biology

A Versatile Model of Hard Tick Infestation on Laboratory Rabbits

Consuelo Almazán¹, Sarah Bonnet¹, Martine Cote¹, Mirko Slovák², Yoonseong Park³, Ladislav Šimo¹

¹Unité Mixte de Recherche (UMR) Biologie moléculaire et immunologie parasitaires (BIPAR), Institut national de la recherche agronomique (INRA), Ecole Nationale Vétérinaire d'Alfort, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est, ²Institute of Zoology, Slovak Academy of Sciences, ³Department of Entomology, Kansas State University

We have developed a simple and versatile system to feed hard ticks on laboratory rabbits. Our non-laborious protocol uses easily accessible materials and can be adjusted depending on the requirements of the various experimental settings. The method allows comfortable monitoring and/or sampling of ticks during the entire feeding period.

Biology

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Lourdes Mateos-Hernández¹, Sabine Rakotobe¹, Baptiste Defaye^1,2,3, Alejandro Cabezas-Cruz¹, Ladislav Šimo¹

¹UMR BIPAR, INRAE, Ecole Nationale Vétérinaire d'Alfort, ANSES, Université Paris-Est, ²Faculté de Pharmacie, Université de Limoges, ³UMR SPE 6134 CNRS, Université de Corte Pascal Paoli

In this study, a feeding system for nymphal and larval stages of hard tick was developed using a capsule attached to laboratory mouse. The feeding capsule is made from flexible materials and remains firmly attached to the mouse for at least one week and allows comfortable monitoring of tick feeding.