Max Planck Institute of Psychiatry
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
Marc Bettscheider 1, Arleta Kuczynska 1, Osborne Almeida 1, Dietmar Spengler 1
1Max Planck Institute of Psychiatry
A streamlined workflow to study DNA methylation and gene expression changes upon early-life stress is shown. Starting from maternal separation of newborn mice and isolation of discrete brain tissues, we represent a protocol to simultaneously isolate DNA and RNA from brain tissue punches for subsequent bisulfite sequencing and RT-PCR analysis.
Behavior
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
Alessandro F. Ulivi 1, Tim P. Castello-Waldow 1, Ghabiba Weston 1,2, Long Yan 3, Ryohei Yasuda 3, Alon Chen 1,4, Alessio Attardo 1
1Dept. of Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, 2Graduate School of Systemic Neurosciences, Ludwig Maximilians University, 3Max Planck Florida Institute for Neuroscience, 4Dept. of Neurobiology, Weizmann Institute of Science
This method describes a chronic preparation that allows optical access to the hippocampus of living mice. This preparation can be used to perform longitudinal optical imaging of neuronal structural plasticity and activity-evoked cellular plasticity over a period of several weeks.
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