Raymond and Beverly Sackler Foundation

2 ARTICLES PUBLISHED IN JoVE

Medicine

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Chung Wong¹, Evan Vosburgh^1,2, Arnold J. Levine^2,3, Lei Cong², Eugenia Y. Xu^1,2

¹Raymond and Beverly Sackler Foundation, ²The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, ³School of Natural Sciences, Institute for Advanced Study, Princeton, New Jersey

We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.

Biology

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

Wenjin Chen^2,3, Chung Wong^1,3, Evan Vosburgh^1,3, Arnold J. Levine^3,4, David J. Foran^2,3, Eugenia Y. Xu^1,3

¹Raymond and Beverly Sackler Foundation, New Jersey, ²Histopathology and Imaging Shared Resource, Rutgers University, ³Rutgers Cancer Institute of New Jersey, Rutgers University, ⁴School of Natural Sciences, Institute for Advanced Study, New Jersey

We present a high-throughput image analysis software application to measure the size of three-dimensional tumor spheroids imaged with bright-field microscopy. This application provides a fast and effective way to examine the effects of therapeutic drugs on spheroids, which is beneficial for researchers who wish to use spheroids in drug screens.