Balgrist University Hospital, Zurich

2 ARTICLES PUBLISHED IN JoVE

Medicine

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

Matthias J.E. Arlt¹, Walter Born¹, Bruno Fuchs¹

¹Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich

The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.

Medicine

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Daniela Meier^*1, Carmen Campanile^*1, Sander M. Botter¹, Walter Born¹, Bruno Fuchs¹

¹Laboratory for Orthopedic Research, Balgrist University Hospital, Zurich, Switzerland

The protocol reported here allows the evaluation of the efficacy of photodynamic therapy (PDT) in cell lines and the optimization of the PDT settings before applying the therapy in animal models.