Keck School of Medicine of the University of Southern California

6 ARTICLES PUBLISHED IN JoVE

Bioengineering

Tissue Engineering of the Intestine in a Murine Model

Erik R. Barthel¹, Allison L. Speer¹, Daniel E. Levin¹, Frédéric G. Sala¹, Xiaogang Hou¹, Yasuhiro Torashima¹, Clarence M. Wigfall¹, Tracy C. Grikscheit¹

¹Children's Hospital Los Angeles, Division of Pediatric Surgery, Saban Research Institute, Keck School of Medicine of the University of Southern California

This article and the accompanying video present our protocol for generating tissue-engineered intestine in the mouse, using an organoid units-on-scaffold approach.

Medicine

Whole Vitreous Humor Dissection for Vitreodynamic Analysis

Karthik Murali¹, Amir H. Kashani², Mark S. Humayun¹

¹Biomedical Engineering, University of Southern California, ²Ophthalmology, University of Southern California

The goal of this protocol is to show an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes.

Developmental Biology

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

Ksenia Gnedeva^1,2, A. J. Hudspeth³, Neil Segil^1,2

¹Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, ²Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, ³Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University

Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.

Neuroscience

Retinal Vascular Reactivity as Assessed by Optical Coherence Tomography Angiography

Sam Kushner-Lenhoff¹, Bright S. Ashimatey¹, Amir H. Kashani^1,2

¹Department of Ophthalmology, USC Roski Eye Institute, ²USC Ginsberg Institute for Biomedical Therapeutics, Keck School of Medicine of the University of Southern California

This article describes a method for measuring retinal vasculature reactivity in vivo with human subjects using a gas breathing provocation technique to deliver vasoactive stimuli while acquiring retinal images.

Cancer Research

Aqueous Humor as a Liquid Biopsy for Retinoblastoma: Clear Corneal Paracentesis and Genomic Analysis

Mary E. Kim^1,2, Liya Xu^1,2,3,11, Rishvanth K. Prabakar⁴, Lishuang Shen⁵, Chen-Ching Peng^1,3, Peter Kuhn^3,6,7,8, Xiaowu Gai^5,9, James Hicks^3,6,10, Jesse L. Berry^1,2,6,11

¹The Vision Center, Children’s Hospital Los Angeles, ²USC Roski Eye Institute, Keck School of Medicine of the University of Southern California, ³Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, University of Southern California, ⁴Department of Molecular and Computational Biology, University of Southern California, ⁵Center for Personalized Medicine, Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, ⁶Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, ⁷Department of Aerospace and Mechanical Engineering, Viterbi School of Engineering, University of Southern California, ⁸Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, ⁹Department of Pathology and Laboratory Medicine, Keck School of Medicine of USC, ¹⁰Department of Biochemistry and Molecular Medicine, Keck School of Medicine, University of Southern California, ¹¹The Saban Research Institute, Children’s Hospital Los Angeles

The aqueous humor is a high-yield liquid biopsy for retinoblastoma, intraocular cancer that cannot be biopsied in vivo due to the risk of extraocular spread. Herein, a method for safely extracting aqueous humor via clear corneal paracentesis and steps for genomic analysis to identify prognostic biomarkers are presented.

Medicine

Development of a Surgical Technique for Subretinal Implants in Rats

Juan Carlos Martinez Camarillo^1,2, Yuntao Hu^1,5, Biju B. Thomas^1,2, Danhong Zhu³, David R. Hinton^2,3, Debbie Mitra^1,2, Jose Miguel Mora Correa², Deepthi S. Rajendran Nair^1,2, Jane Lebkowski⁴, Mark S. Humayun^1,2

¹USC Roski Eye Institute, Keck School of Medicine of the University of Southern California, ²USC Dr. Allen and Charlotte Ginsburg Institute for Biomedical Therapeutics, University of Southern California, ³Department of Pathology, Keck School of Medicine of the University of Southern California, ⁴Regenerative Patch Technologies LLC, ⁵Eye center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University

The present protocol describes the scleral approach for subretinal device implantation, a feasible surgical technique for implementation in animal models of retinal diseases in research.