Shanghai University

7 ARTICLES PUBLISHED IN JoVE

Cancer Research

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Xiaoxing Lv¹, Meiru Zhao¹, Yuting Yi¹, Lucheng Zhang¹, Yanfang Guan¹, Tao Liu¹, Ling Yang¹, Rongrong Chen¹, Jianhui Ma¹, Xin Yi¹

¹Geneplus-Beijing Institute

This manuscript describes a technique for detecting mutations of low frequency in ctDNA, ER-Seq. This method is differentiated by its unique use of two-directional error correction, a special background filter, and efficient molecular acquirement.

Engineering

Radio Frequency Magnetron Sputtering of GdBa₂Cu₃O₇₋_δ/ La_0.67Sr_0.33MnO₃ Quasi-bilayer Films on SrTiO₃ (STO) Single-crystal Substrates

Ying Wang^1,2, Zhen Li^1,2, YongSheng Liu¹, Yijie Li², Linfei Liu², Da Xu², Xiaojing Luo¹, Tian Gao^1,3, Yanyan Zhu^1,4, Luozeng Zhou³, Jianming Xu³

¹Department of Physics, Mathematics, Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power, Shanghai University of Electric Power, ²Key Laboratory of Artificial Structure and Quantum Control, Ministry of Education, Department of Physics, Shanghai Jiao Tong University, ³Shanghai Institute of Space Power-sources, ⁴Shanghai Key Laboratory of High Temperature Superconductors, Shanghai University

Here, we present a protocol to grow LSMO nanoparticles and (Gd) BCO films on (001) SrTiO₃ (STO) single-crystal substrates by radio frequency (RF)-sputtering.

Neuroscience

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Mengye Zhu¹, Daying Zhang¹, Sicong Peng², Nana Liu², Jing Wu², Haixia Kuang², Tao Liu^2,3

¹Department of Pain, First Affiliated Hospital of Nanchang University, ²Department of Pediatrics, First Affiliated Hospital of Nanchang University, ³Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.

Medicine

OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery

Tao Liu^*1,2,3,4,5, Xueling Song^*1,2,3,4,5, Xiaoying Zheng^*1,2,3,4,5, Liying Yan^1,2,3,4,5, Caihong Ma^1,2,3,5, Rong Li^1,2,3,4,5, Jie Yan^1,2,3,4,5, Jie Qiao^1,2,3,4,5

¹Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, ²National Clinical Research Center for Obstetrics and Gynecology, ³Key Laboratory of Assisted Reproduction, Ministry of Education, ⁴Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, ⁵Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences

In vitro maturation (IVM) before gynecological operation (OP-IVM) combines IVM following oocyte retrieval with routine gynecological surgery and serves as an extension of conventional IVM applications for fertility preservation.

Behavior

Usability Evaluation of Augmented Reality: A Neuro-Information-Systems Study

Jun Wu^*1, Di Zhang^*1, Tao Liu^2,3,4, Helen Hong Yang⁵, Yi Wang¹, Huili Yao¹, Shinan Zhao¹

¹Economics and Management School, Jiangsu University of Science and Technology, ²School of Health, Fujian Medical University, ³School of Management, Shanghai University, ⁴School of Management, Zhejiang University, ⁵Business School, La Trobe University

This study presents an experimental paradigm for a usability test combining subjective and objective evaluations. The objective evaluation adopted Neuro-Information-Systems (NeuroIS) methods, and the subjective evaluation adopted a usability questionnaire and a NASA-Task Load Index (NASA-TLX) scale.

Innovative Animal Models Of Cardiac Remodeling: Development And Evaluation

Feng Hua Yang¹, W. Glen Pyle^2,3, Yihua Bei⁴

¹Cardiovascular Model Research Center, Guangdong Laboratory Animals Monitoring Institute, ²IMPART Investigator Team, Dalhousie Medicine, ³Department of Biomedical Sciences, University of Guelph, ⁴School of Medicine, Shanghai University

Innovative Animal Models Of Cardiac Remodeling: Development And Evaluation

Bioengineering

Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

Yang Li^*1,2,3, Yue Wang^*4, Jun Yin³, Jin Qian^1,2

¹School of Aeronautics and Astronautics, Zhejiang University, ²Department of Engineering Mechanics, Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province, Zhejiang University, ³The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, ⁴Institute of Artificial Intelligence, School of Future Technology, Shanghai University

This study uses temperature and material composition to control the yield stress properties of yield stress fluids. The solid-like state of the ink can protect the printing structure, and the liquid-like state can continuously fill the printing position, realizing the digital light processing 3D printing of extremely soft bioinks.