Los Alamos National Laboratory

4 ARTICLES PUBLISHED IN JoVE

Biology

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Miriam Cohen¹, Nissi M. Varki¹, Mark D. Jankowski², Pascal Gagneux¹

¹Department of Cellular and Molecular Medicine, University of California, San Diego , ²Biosecurity and Public Health, Los Alamos National Laboratory

Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

Education

Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles

Saeed Mozaffari^*1, Wenhui Li^*1, Coogan Thompson¹, Sergei Ivanov², Soenke Seifert³, Byeongdu Lee⁴, Libor Kovarik⁵, Ayman M. Karim¹

¹Department of Chemical Engineering, Virginia Polytechnic Institute and State University, ²Center for Integrated Nanotechnologies, Los Alamos National Laboratory, ³Advanced Photon Source, Argonne National Laboratory, ⁴X-ray Science Division, Argonne National Laboratory, ⁵Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory

The main goal of this work is to elucidate the role of capping agents in regulating the size of palladium nanoparticles by combining in situ small angle x-ray scattering (SAXS) and ligand-based kinetic modeling.

Engineering

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Trevor Clark¹, Caitlin A. Taylor^1,2, Christopher M. Barr¹, Khalid Hattar¹

¹Sandia National Laboratories, ²Los Alamos National Laboratory

Sample preparation techniques are outlined with specific considerations for in situ ion irradiation TEM experiments. Ion species, energy, and fluence are discussed with methods for how to select and compute them. Finally, procedures for conducting an experiment are described and accompanied by the representative results.

Medicine

Randomized Controlled Trial to Study the Acute Effects of Strength Exercise on Insulin Sensitivity in Obese Adults

Luis Filipe Rocha Silva¹, Bruna Caroline Chaves Garcia², Zach A. Mang³, Fabiano Trigueiro Amorim⁴, Marco Fabrício Dias-Peixoto¹, Fernando Gripp¹, Valmor Tricoli⁵, Flávio de Castro Magalhães^1,4

¹Department of Physical Education, Federal University of the Jequitinhonha and Mucuri Valleys, ²Laboratory of Exercise Biology and Immunometabolism, Centro Integrado de Pós-Graduação e Pesquisa em Saúde, Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, Federal University of the Jequitinhonha and Mucuri Valleys, ³Occupational Safety & Health, Los Alamos National Laboratory, ⁴Department of Health, Exercise, and Sports Sciences, University of New Mexico, ⁵School of Physical Education and Sport, University of Sao Paulo

This study describes a randomized controlled trial protocol aiming at assessing the acute effects of strength exercise volume on insulin sensitivity in obese individuals.