PerkinElmer, Inc.

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography

Elza van Deel^1,2, Yanto Ridwan¹, J. Nicole van Vliet¹, Sasha Belenkov³, Jeroen Essers^1,4,5

¹Department of Genetics, Erasmus MC, Rotterdam, ²Department of Experimental Cardiology, Erasmus MC, Rotterdam, ³PerkinElmer, Inc., ⁴Department of Vascular Surgery, Erasmus MC, Rotterdam, ⁵Department of Radiation Oncology, Erasmus MC, Rotterdam

This method outlines the use of Quantum Micro-Computed Tomography (MicroCT) to assess cardiac morphology, function, perfusion, metabolism and viability with iodinated contrast agent in mice with experimentally-induced myocardial ischemia. The technique can be applied for non-destructive high-throughput longitudinal in vivo imaging of various animal models of human heart disease.