Burnet Institute

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Thomas A. Angelovich^1,2, Anna C. Hearps^1,3, Anna Maisa¹, Theodoros Kelesidis⁴, Anthony Jaworowski^1,3

¹Centre for Biomedical Research, Burnet Institute, ²School of Health and Biomedical Sciences, RMIT University, ³Department of Infectious Diseases, Monash University, ⁴University of California, Los Angeles

We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

Immunology and Infection

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Shubhendu Sen Roy¹, Huy Cong Xuan Nguyen¹, Thomas A. Angelovich^2,3, Anna C. Hearps², Diana Huynh^1,4, Anthony Jaworowski^2,4, Theodoros Kelesidis¹

¹University of California, Los Angeles, ²Centre for Biomedical Research, Burnet Institute, ³School of Health and Biomedical Sciences, RMIT University, ⁴Department of Infectious Diseases, Monash University

We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

Chemistry

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Miguela Martin¹, David Perez-Guaita¹, Dean W. Andrew³, Jack S. Richards^3,4, Bayden R. Wood¹, Philip Heraud^1,2

¹Centre for Biospectroscopy, Monash University, ²Department of Microbiology, Faculty of Medicine, Nursing and Health Sciences, Monash University, ³Centre for Biomedical Research, Burnet Institute, ⁴Department of Medicine, University of Melbourne

Here, we present a protocol for the detection and quantification of Plasmodium falciparum in infected aqueous red blood cells using an attenuated total reflection infrared spectrometer and multivariate data analysis.