SCIREQ Scientific Respiratory Equipment Inc.

3 ARTICLES PUBLISHED IN JoVE

Biology

Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique

Toby K. McGovern^*1, Annette Robichaud^*2, Liah Fereydoonzad², Thomas F. Schuessler², James G. Martin¹

¹Meakins-Christie Laboratories, Department of Medicine, McGill University, ²SCIREQ Scientific Respiratory Equipment Inc.

The present protocol provides a detailed step-by-step description of the procedures required to execute measurements of respiratory system mechanics as well as the assessment of airway responsiveness to inhaled methacholine in mice using the forced oscillation technique (flexiVent; SCIREQ Inc, Montreal, Qc, Canada).

Medicine

Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography

Samuel Mailhot-Larouche¹, Louis Deschênes¹, Katherine Lortie¹, Morgan Gazzola¹, David Marsolais¹, David Brunet², Annette Robichaud², Ynuk Bossé¹

¹Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, ²SCIREQ Scientific Respiratory Equipment Inc.

The objective of the present article is to provide a detailed description of the recommended procedures to evaluate respiratory function in conscious mice by double-chamber plethysmography.

Chemistry

Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies

Alexandra Noël¹, Christina M. Verret¹, Farhana Hasan², Slawomir Lomnicki², John Morse³, Annette Robichaud³, Arthur L. Penn¹

¹Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, ²Department of Environmental Sciences, College of the Coast & Environment, Louisiana State University, ³SCIREQ Scientific Respiratory Equipment Inc.

Electronic cigarette (e-cig) users are increasing worldwide. Little, however, is known about the health effects induced by inhaled e-cig aerosols. This article describes an e-cig aerosol generation technique suitable for animal exposures and subsequent toxicological studies. Such protocols are required to establish experimentally reproducible and standardized e-cig exposure systems.