VA Greater Los Angeles Healthcare System
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants
Mauro W. Zappaterra 1, Anthony S. LaMantia 2, Christopher A. Walsh 3,4, Maria K. Lehtinen 5
1Department of Physical Medicine and Rehabilitation, VA Greater Los Angeles Healthcare System, 2Department of Pharmacology and Physiology, Institute for Neuroscience, The George Washington University School of Medicine and Health Sciences, 3Division of Genetics, Department of Medicine, Boston Children's Hospital, 4Howard Hughes Medical Institute, Boston Children's Hospital, 5Department of Pathology, Boston Children's Hospital, Harvard Medical School
The ventricular cerebrospinal fluid (CSF) bathes the neuroepithelial and cerebral cortical progenitor cells during early brain development in the embryo. Here we describe the method developed to isolate ventricular CSF from rodent embryos of different ages in order to investigate its biological function. In addition, we demonstrate our cerebral cortical explant dissection and culture technique that allows for explant growth with minimal volumes of culture medium or CSF.
Biology
Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis
Richard L. Watson 1,2, Wing Yi Lung 1, Steven J. Bensinger 3, John A. Belperio 1
1Department of Medicine, Division of Pulmonary & Critical Care, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Medicine, Pulmonary & Critical Care Section, VA Greater Los Angeles Healthcare System, 3Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles
This protocol demonstrates the oropharyngeal aspiration technique for use in the bleomycin murine model of pulmonary fibrosis.
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