Korea Institute of Science and Technology (KIST)

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

Mina Lee^1,2, Dongwook Kim^1,2, Hee-Sup Shin^1,2, Ho-Geun Sung³, Jee Hyun Choi^1,2

¹Center for Neural Science , Korea Institute of Science and Technology (KIST), ²Department of Neuroscience, University of Science and Technology, ³Fab Service Department, Korea Advanced Nano Fab Center

In this article, we described the surgery procedure and handling tips for implantation of ultra-thin polyimide-based microelectrode array (PBM-array) on the mouse skull for acquisition of high-density encephalography (EEG) in a mouse model.

Education

Magnetic Tweezers for the Measurement of Twist and Torque

Jan Lipfert¹, Mina Lee¹, Orkide Ordu¹, Jacob W. J. Kerssemakers¹, Nynke H. Dekker¹

¹Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology

Magnetic tweezers, a powerful single-molecule manipulation technique, can be adapted for the direct measurements of the twist (using a configuration called freely-orbiting magnetic tweezers) and torque (using a configuration termed magnetic torque tweezers) in biological macromolecules. Guidelines for performing such measurements are given, including applications to the study of DNA and associated nucleo-protein filaments.

Biochemistry

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Md Nazmul Huda^1,2, Erdenedolgor Erdene-Ochir^1,2, Cheol-Ho Pan^1,2

¹Systems Biotechnology Research Center, KIST Gangneung Institute of Natural Products, ²Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology

This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.

Biochemistry

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Dohwan Lee^*1, Kyung Wook Wee^*1, Hyerin Kim^*1, Sung Il Han¹, Seungmin Kwak^1,2, Dae Sung Yoon³, Jeong Hoon Lee¹

¹Department of Electrical Engineering, Kwangwoon University, ²Korea Institute of Science and Technology (KIST), ³School of Biomedical Engineering, Korea University

Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes.

Biochemistry

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Sayan Dutta Gupta¹, Dae-Geun Song¹, SueJung Lee¹, Jae Wook Lee¹, Jin-Soo Park¹, Chrisostomos Prodromou², Cheol-Ho Pan¹

¹Gangneung Institute of Natural Products, Korea Institute of Science and Technology (KIST), ²School of Life Sciences, University of Sussex

The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes.