University Medicine Greifswald

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes

Lars G. Eckerle¹, Stephan B. Felix¹, Lars R. Herda¹

¹Department of Internal Medicine B, University Medicine Greifswald

The presented method offers a way to detect functional effective cardiotropic autoantibodies in the plasma of patients with dilated cardiomyopathy, irrespective of the specific antigen, by analysing the impact of isolated patient immunoglobulin on cellular shortening and intracellular calcium transients in isolated rat cardiomyocytes.

Behavior

Visualization of Intensity Levels to Reduce the Gap Between Self-Reported and Directly Measured Physical Activity

Lisa Voigt^1,4, Antje Ullrich^1,4, Ulrike Siewert-Markus^1,2,4, Marcus Dörr^3,4, Ulrich John^1,4, Sabina Ulbricht^1,4

¹Institute of Social Medicine and Prevention, University Medicine Greifswald, ²Institute for Medical Psychology, University Medicine Greifswald, ³Department of Internal Medicine B, University Medicine Greifswald, ⁴partner site Greifswald, DZHK (German Centre for Cardiovascular Research)

This protocol describes a randomized controlled trial as a method to test the effect of a video demonstration on the intra-individual difference between self-reported and accelerometer-based moderate-to-vigorous physical activity.

Neuroscience

Neuronavigated Focalized Transcranial Direct Current Stimulation Administered During Functional Magnetic Resonance Imaging

Filip Niemann^*1, Alireza Shahbabaie^*1, Sven Paßmann¹, Steffen Riemann¹, Robert Malinowski¹, Harun Kocataş¹, Leonardo M. Caisachana Guevara^1,2, Mohamed Abdelmotaleb¹, Daria Antonenko¹, Felix Blankenburg^3,4, Rico Fischer², Gesa Hartwigsen^5,6, Shu-Chen Li^7,8, Michael A. Nitsche^9,10,11, Axel Thielscher^12,13, Dagmar Timmann¹⁴, Anna Fromm¹, Dayana Hayek¹, Ann-Kathrin Hubert¹, Andrew K. Martin^15,16, Alexander Hunold¹⁷, Agnes Flöel^*1,18, Marcus Meinzer^*1

¹Department of Neurology, University Medicine Greifswald, ²Department of Psychology, University Medicine Greifswald, ³Berlin School of Mind and Brain, Humboldt Universität zu Berlin, ⁴Neurocomputation and Neuroimaging Unit, Freie Universität Berlin, ⁵Wilhelm-Wundt-Institute for Psychology, Cognitive and Biological Psychology, Leipzig University, ⁶Lise Meitner Research Group Cognition and Plasticity, Max Planck Institute for Human Cognitive and Brain Sciences, ⁷Lifespan Developmental Neuroscience, Faculty of Psychology, Technische Universität Dresden, ⁸Centre for Tactile Internet with Human-in-the-Loop, Technische Universität Dresden, ⁹Department of Psychology and Neurosciences, Leibniz Research Centre for Working Environment and Human Factors, ¹⁰German Centre for Mental Health (DZPG), ¹¹Clinic of Psychiatry and Psychotherapy, Protestant Hospital of Bethel Foundation, University Hospital OWL, Bielefeld University, ¹²Danish Research Centre for Magnetic Resonance, Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital Amager and Hvidovre, ¹³Department of Health Technology, Technical University of Denmark, ¹⁴Department of Neurology and Center for Translational Neuro- and Behavioral Sciences (C-TNBS), Essen University Hospital, ¹⁵Department of Psychology, University of Kent, ¹⁶Kent Medway Medical School, ¹⁷Institute of Biomedical Engineering and Informatics, Technische Universität Ilmenau, ¹⁸German Center for Neurodegenerative Diseases (DZNE Site Greifswald)

This protocol describes the method of neuronavigated electrode placement for focal, transcranial direct current stimulation (tDCS) administered during functional magnetic resonance imaging (fMRI).

Cancer Research

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Maryna Somova¹, Marlene Grosse¹, Frank Schulze², Martin Burchardt¹, Pedro Caetano Pinto¹

¹Department of Urology, University Medicine Greifswald, ²Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald

Here, we present protocols that detail instructions for implementing 3D cell cultures using collagen and collagen-agarose matrixes in a microphysiological system. These protocols support renal proximal tubule and renal cell carcinoma spheroids co-culture, simulating in vivo conditions and enabling advanced investigation of kidney cancer cell interactions.