Guizhou Medical University

7 ARTICLES PUBLISHED IN JoVE

Neuroscience

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

Aoshuang Chang^1,2, Cuixian Li¹, Jianfeng Huang^1,3, Wenlu Pan¹, Yinghong Tian⁴, Jie Tang¹

¹Department of Physiology, School of Basic Medical Sciences, Southern Medical University, ²School of Basic Medical Sciences, Guizhou Medical University, ³Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region, Southern Medical University, ⁴Experiment Teaching Center, School of Basic Medical Sciences, Southern Medical University

This protocol describes methods to record the auditory brainstem response from postnatal rat pups. To examine the functional development of outer hair cells, the experimental procedure of whole-cell patch clamp recording in isolated outer hair cells is described step-by-step.

Immunology and Infection

Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines

Lingling Chen^*1,2, Qiuqiu Xiao^*1, Mengting Shi¹, Jinzhi Cheng¹, Jiahong Wu¹

¹Guizhou Medical University, ²Guizhou Health Vocational College

Four methods were used to detect intracellular Wolbachia, which complemented each other and improved the detection accuracy of Wolbachia infection of Aedes albopictus-derived Aa23 and Aa23-T cured of native Wolbachia infection using antibiotics.

Biology

Control of Aedes albopictus Mosquito Larvae with Carpesium abrotanoides L.

Qiuguo Liang^1,2, Xi Yang^1,2, Dejun Liao^1,2, Cheng Yi^1,2, Rongmin Dang^1,2, Jiahong Wu³, Lin Huang^1,2

¹Qiannan Medical College for Nationalities, ²Key Laboratory of Human Parasitic Diseases in Qiannan Prefecture, ³Guizhou Medical University

Using the Carpesium abrotanoides L. plant for mosquito larvae control can effectively reduce the Aedes albopictus mosquito population and provide a foundation for designing plant-derived insecticides.

Cancer Research

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer

Dingwen Xu^*1, Xiaofei Hu^*2, Yang Feng¹, Zhihua Lu¹, Xianmei Liu³, Huishen Yan¹, Jianming Peng¹, Sen Luo¹, Weijuan Yao⁴

¹Department of Clinic, School of Medicine, Yangzhou Polytechnic College, ²Department of Nuclear Medicine, Huangshi Central Hospital, Affiliated Hospital of Hubei Institute of Technology, ³School of Biology and Engineering, Guizhou Medical University, ⁴Hemorheology Center, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center

Tropomodulin 3 (TMOD3) has been increasingly studied in tumors in recent years. This study is the first to report that TMOD3 is highly expressed in ovarian cancer and is closely associated with platinum resistance and immune infiltration. These results could help improve the therapeutic outcomes for ovarian cancer.

Biology

Generating the Transcriptional Regulation View of Transcriptomic Features for Prediction Task and Dark Biomarker Detection on Small Datasets

Kewei Li¹, Yusi Fan¹, Yaqing Liu¹, Hongmei Liu², Gongyou Zhang², Meiyu Duan¹, Lan Huang¹, Fengfeng Zhou¹

¹College of Computer Science and Technology, and Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education, Jilin University, ²School of Biology and Engineering, Guizhou Medical University

Here, we introduce a protocol for converting transcriptomic data into a mqTrans view, enabling the identification of dark biomarkers. While not differentially expressed in conventional transcriptomic analyses, these biomarkers exhibit differential expression in the mqTrans view. The approach serves as a complementary technique to traditional methods, unveiling previously overlooked biomarkers.

Medicine

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Xiaoyu Tian^*1,2,3, Meng Zhang^*1,2, Yiming Wu⁴, Liying Zhang^1,2, Lingli Zhang^1,2, Xueer Zheng^1,2, Shangkun Ou^1,2, Hao Gu^1,2

¹Department of Ophthalmology, the Affiliated Hospital of Guizhou Medical University, ²School of Clinical Medicine, Guizhou Medical University, ³The Second People's Hospital of Guiyang City, ⁴Department of Biomedical Sciences, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham

We developed a rat model of severe corneal inflammation through corneal epithelium curettage combined with corneal sutures. The study evaluated corneal inflammation patterns, epithelial proliferation, and changes in limbal stem cells under inflammatory conditions.

Medicine

Establishment of A Mouse Model of Aqueous Deficiency Dry Eye

Meng Zhang^*1,2, Xiaoyu Tian^*1,2, Yiming Wu³, Liying Zhang^1,2, Lingli Zhang^1,2, Xueer Zheng^1,2, Shangkun Ou^1,2, Hao Gu^1,2

¹Department of Ophthalmology, the Affiliated Hospital of Guizhou Medical University, ²School of Clinical Medicine, Guizhou Medical University, ³Department of Biomedical Sciences, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham

Here, we present a method to establish a mouse model of aqueous-deficient dry eye by excising the extraorbital and infraorbital lacrimal glands and evaluate the changes in the ocular surface in aqueous deficiency dry eye.