University Hospital of Bonn

3 ARTICLES PUBLISHED IN JoVE

Medicine

A Model to Simulate Clinically Relevant Hypoxia in Humans

Lars Eichhorn¹, Florian Kessler¹, Volker Böhnert², Felix Erdfelder¹, Anja Reckendorf³, Rainer Meyer⁴, Richard K. Ellerkmann¹

¹Department of Anaesthesiology and Intensive Care Medicine, University Hospital of Bonn, ²Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, ³Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, ⁴Institute of Physiology 2, University of Bonn

Hypoxia simulation in humans has usually been performed by inhaling hypoxic gas mixtures. For this study, apneic divers were used to simulate dynamic hypoxia in humans. Additionally, physiological changes in desaturation and re-saturation kinetics were evaluated with non-invasive tools such as Near-Infrared-Spectroscopy (NIRS) and peripheral oxygenation saturation (SpO₂).

Medicine

A Closed-chest Model to Induce Transverse Aortic Constriction in Mice

Lars Eichhorn^*1, Christina Katharina Weisheit^*1, Christopher Gestrich², Konrad Peukert¹, Georg Daniel Duerr², Muhammad Ajmal Ayub³, Felix Erdfelder¹, Florian Stöckigt⁴

¹Department of Anaesthesiology, University Hospital Bonn, ²Department of Cardiac Surgery, University Hospital Bonn, ³Institute of Physiology 2, University of Bonn, ⁴Department of Medicine - Cardiology, University Hospital Bonn

Here, we present a protocol of Transverse Aortic constriction (TAC) via a lateral thoracotomy. This technique is a minimally invasive, closed chest surgical procedure aiming to simulate pressure overload and heart failure in mice utilizing standard TAC laboratory settings.

Medicine

Vitrification of Ovarian Cortex Tissue to Achieve a Glassy State of Aggregation

Andreas Schallmoser¹, Julia John¹, Rebekka Einenkel^*1, Nicole Sänger^*1

¹Department of Gynecological Endocrinology and Reproductive Medicine, University Hospital of Bonn

A protocol for the vitrification of ovarian tissue, as an alternative cryopreservation method to the widely used slow freezing protocol, is presented.