Sannio University

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Chiara Pagliuca¹, Elena Scaglione¹, Francesca Carraturo¹, Giuseppe Mantova¹, Maria Michela Marino¹, Maria Virginia Pishbin¹, Caterina Pagliarulo², Roberta Colicchio¹, Paola Salvatore^1,3

¹Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, ²Department of Science and Technology, Sannio University, ³CEINGE – Advanced Biotechnology

Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.