Helmholtz-Zentrum Berlin

3 ARTICLES PUBLISHED IN JoVE

Biochemistry

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Christian G. Feiler¹, Dirk Wallacher², Manfred S. Weiss¹

¹Macromolecular Crystallography (HZB-MX), Helmholtz-Zentrum Berlin, ²Department Sample Environment, Helmholtz-Zentrum Berlin

A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.

Biochemistry

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Jan Wollenhaupt¹, Tatjana Barthel^1,2, Gustavo M. A. Lima³, Alexander Metz⁴, Dirk Wallacher⁵, Elmir Jagudin³, Franziska U. Huschmann^1,4, Thomas Hauß¹, Christian G. Feiler¹, Martin Gerlach¹, Michael Hellmig¹, Ronald Förster¹, Michael Steffien¹, Andreas Heine⁴, Gerhard Klebe⁴, Uwe Mueller¹, Manfred S. Weiss¹

¹Macromolecular Crystallography, Helmholtz-Zentrum Berlin, ²Structural Biochemistry Group, Institute for Chemistry and Biochemistry, Freie Universität Berlin, ³BioMAX, MAX IV Laboratory, ⁴Drug Design Group, Institute of Pharmaceutical Chemistry, Philipps-Universität Marburg, ⁵Department Sample Environment, Helmholtz-Zentrum Berlin

Crystallographic fragment screening at the Helmholtz-Zentrum Berlin is performed using a workflow with dedicated compound libraries, crystal handling tools, fast data collection facilities and largely automated data analysis. The presented protocol intends to maximize the output of such experiments to provide promising starting points for downstream structure-based ligand design.

iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology

Hans Wienk¹, Lucia Banci², Susan Daenke³, Eva Pereiro⁴, Harald Schwalbe⁵, David Ian Stuart⁶, Manfred S. Weiss⁷, Anastassis Perrakis¹

¹Division of Biochemistry and Oncode Institute, The Netherlands Cancer Institute, Amsterdam, ²CERM/CIRMMP, University of Florence, ³Instruct-ERIC, Oxford University, ⁴MISTRAL beamline, Alba Light Source, ⁵Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, ⁶Division of Structural Biology, Oxford University, ⁷Macromolecular Crystallographyy, Helmholtz-Zentrum Berlin

iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology