University of California at Riverside
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
Xiang Li 1, Samantha Marie Mearns 1, Manuela Martins-Green 2, Yuxin Liu 1
1Lane Department of Computer Science and Electrical Engineering, West Virginia University, 2Department of Cell Biology and Neuroscience, University of California at Riverside
A microchannels-on-a-chip platform was developed by the combination of photolithographic reflowable photoresist technique, soft lithography, and microfluidics. The endothelialized microchannels platform mimics the three-dimensional (3D) geometry of in vivo microvessels, runs under controlled continuous perfusion flow, allows for high-quality and real-time imaging and can be applied for microvascular research.
Medicine
Protocol to Create Chronic Wounds in Diabetic Mice
Jane Hannah Kim 1, Manuela Martins-Green 1
1Department of Molecular, Cell and Systems Biology, University of California, Riverside
Chronic wounds are developed from acute wounds on a diabetic mouse model by inducing high levels of oxidative stress after a full-thickness cutaneous wound. The wound is treated with inhibitors for catalase and glutathione peroxidase, resulting in impaired healing and biofilm development by bacteria present in the skin microbiome.
