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Institut Pasteur, Paris, France

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Charlotte Keller *1, Nora Mellouk *1, Anne Danckaert 2, Roxane Simeone 3, Roland Brosch 3, Jost Enninga 1, Alexandre Bobard 1
1Dynamique des Interactions Hôte Pathogène, Institut Pasteur, Paris, France, 2Imagopole, Institut Pasteur, Paris, France, 3Pathogenomique Mycobacterienne Integrée, Institut Pasteur, Paris, France

We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.

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Immunology and Infection

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
Fabio Cafini *1,2, Nguyen Thi Le Thuy *3, Federico Román 4, José Prieto 5, Sarah Dubrac 6,7, Tarek Msadek 6,7, Kazuya Morikawa 1
1Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, 2Department of Basic Biomedical Science, Universidad Europea de Madrid, 3Human Biology Program, School of Integrative and Global Majors, University of Tsukuba, 4Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa, Instituto de Salud Carlos III, 5Division of Microbiology, Department of Medicine, School of Medicine, Universidad Complutense, 6Biology of Gram-Positive Pathogens, Department of Microbiology, Institut Pasteur, Paris, France, 7ERL3526, CNRS, Paris, France

We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus aureus.

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