KU Leuven and Leuven Institute for Neuroscience and Disease (LIND)
2 ARTICLES PUBLISHED IN JoVE
Education
Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
Jean-Marc Taymans 1, Fangye Gao 1, Veerle Baekelandt 1
1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven and Leuven Institute for Neuroscience and Disease (LIND)
Leucine rich repeat kinases 1 and 2 (LRRK1 and LRRK2) are multidomain proteins which encode both GTPase and kinase domains and which are phosphorylated in cells. Here, we present a protocol to label LRRK1 and LRRK2 in cells with 32P orthophosphate, thereby providing a means to measure their overall cellular phophorylation levels.
Medicine
Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector
Anke Van der Perren 1, Cindy Casteels 2, Koen Van Laere 2, Rik Gijsbers 3,4, Chris Van den Haute 1,4, Veerle Baekelandt 1
1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven, 2Division of Nuclear Medicine, Leuven University Hospital - KU Leuven, 3Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 4Leuven Viral Vector Core, KU Leuven
This manuscript describes how viral vector-mediated local gene delivery provides an attractive way to express transgenes in the central nervous system. The protocol outlines all crucial steps to perform a viral vector injection in the substantia nigra of the rat to develop a viral vector-based animal model for Parkinson's disease.
