Instituto de Salud Carlos III (ISCIII)

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

Alfonso Rubio-Navarro¹, Melania Guerrero-Hue¹, Beatriz Martín-Fernandez², Isabel Cortegano³, Elena Olivares-Alvaro², Natalia de las Heras², Mario Alía³, Belén de Andrés³, María Luisa Gaspar³, Jesús Egido¹, Juan Antonio Moreno¹

¹Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, ²Department of Physiology, Faculty of Medicine, Complutense University, ³Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

This manuscript describes a detailed protocol for phenotypic and quantitative analysis of resident macrophages from rat kidneys by flow cytometry. The resulting stained cells can be also used for other applications, including cell sorting, gene expression analysis or functional studies, thus increasing the information obtained in the experimental model.

Biology

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection

Silvia Murillo-Cuesta^*1,2,3, Néstor Vallecillo^*1, Rafael Cediel^1,2,4, Adelaida M. Celaya^1,2, Luis Lassaletta^3,5, Isabel Varela-Nieto^1,2,3, Julio Contreras^1,2,4

¹Instituto de Investigaciones Biomédicas (IIBm) Alberto Sols CSIC-UAM, ²Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), ³Instituto de Investigación Sanitaria La Paz (IdiPAZ), ⁴Facultad de Veterinaria, Universidad Complutense de Madrid, ⁵Departmento de Otorrino laringología, Hospital Universitario La Paz

Two microsurgery approaches for local drug delivery to the inner ear are described here and compared in terms of impact on hearing parameters, cochlear cytoarchitecture and expression of inflammatory markers.

Medicine

Mobile Device-assisted Dietary Ecological Momentary Assessments for the Evaluation of the Adherence to the Mediterranean Diet in a Continuous Manner

Anna Boronat¹, Joana Clivillé-Pérez², Natalia Soldevila-Domenech^1,2, Laura Forcano^1,3, Nieves Pizarro^1,2, Montserrat Fitó^3,4, Helmut Schröder^4,5, Karine Fauria⁶, Rafael de la Torre^1,2,3

¹Integrative Pharmacology and Systems Neurosciences Research Group, Neurosciences Research Program, Hospital del Mar Medical Research Institute (IMIM), ²Department of Experimental and Health Sciences, University Pompeu Fabra, ³CIBER de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, ⁴Cardiovascular Risk and Nutrition Research Group, Hospital del Mar Medical Research Institute (IMIM), ⁵CIBER de Epidemiología y Salud Pública (CIBERESP), Instituto de Salud Carlos III (ISCIII), ⁶Barcelonaβeta Brain Research Center (BBRC), Pasqual Maragall Foundation

Presented here is a study protocol aimed at monitoring continuous adherence to the Mediterranean diet (MedDiet) by means of ecological momentary assessments. The method evaluates the intake of key food groups of the MedDiet and calculates an index of adherence.