Philip Morris International R&D

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

Diana Kuehn¹, Shoaib Majeed¹, Emmanuel Guedj¹, Remi Dulize¹, Karine Baumer¹, Anita Iskandar¹, Stephanie Boue¹, Florian Martin¹, Radina Kostadinova¹, Carole Mathis¹, Nikolai V. Ivanov¹, Stefan Frentzel¹, Julia Hoeng¹, Manuel C. Peitsch¹

¹Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A.

The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.

Biology

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)

Diego Marescotti¹, Ignacio Gonzalez Suarez¹, Stefano Acali¹, Stephanie Johne¹, Alexandra Laurent¹, Stefan Frentzel¹, Julia Hoeng¹, Manuel C. Peitsch¹

¹Biological System Research (BSR), Philip Morris International R&D

The objective of the study was to assess the biological impact of 15 cigarette smoke constituents using a combination of an impedance-based real time cell analyzer and a high-content screening (HCS)-based platform for toxicological assessment in vitro. This study provides information on effective doses, toxicity and modes of action of the tested compounds.

Engineering

Use of Capillary Aerosol Generator in Continuous Production of Controlled Aerosol for Non-Clinical Studies

Didier Goedertier¹, Sandra Schorderet Weber¹, Francesco Lucci¹, Tom Lee², Wei Teck Tan², Falk Radtke¹, Subash Krishnan², Patrick Vanscheeuwijck¹, Arkadiusz K. Kuczaj^1,3, Julia Hoeng¹

¹PMI R&D, Philip Morris Products S.A., ²PMI R&D, Philip Morris Research Laboratories Pte. Ltd., ³Applied Mathematics, University of Twente

The protocol describes the settings and use of a capillary aerosol generator for continuous production of controlled aerosol from a multispecies liquid solution, suitable for steady large-volume aerosol delivery (e.g., in vivo inhalation studies).