Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain NeuronsJin-Young Koh *1,2, Sadahiro Iwabuchi *1, Zhengmin Huang 3, N. Charles Harata 1
1Department of Molecular Physiology & Biophysics, University of Iowa Carver College of Medicine, 2Department of Psychiatry, University of Iowa Carver College of Medicine, 3EZ BioResearch LLC
We describe procedures for labeling and genotyping newborn mice and generating primary neuronal cultures from them. The genotyping is rapid, efficient and reliable, and allows for automated nucleic-acid extraction. This is especially useful for neonatally lethal mice and their cultures that require prior completion of genotyping.
