Fraunhofer IWS

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Eva-Maria Materne^*1, Ilka Maschmeyer^*1,2, Alexandra K. Lorenz¹, Reyk Horland^1,2, Katharina M. S. Schimek¹, Mathias Busek³, Frank Sonntag³, Roland Lauster¹, Uwe Marx^1,2

¹Medical Biotechnology, Technische Universität Berlin, ²TissUse GmbH, ³Fraunhofer IWS

Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.