Hebei University of Technology

10 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Wei Han¹, Hui Li¹, Brahm H. Segal^2,3, Timothy S. Blackwell¹

¹Department of Medicine, Vanderbilt University School of Medicine, ²Departments of Medicine and Immunology, Roswell Park Cancer Institute, ³Department of Medicine, University at Buffalo School of Medicine

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

Medicine

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens

Kasey Jividen^*1, Mercedeh Javanbakht Movassagh^*1, Amir Jazaeri², Hui Li¹

¹Department of Pathology, University of Virginia, ²Department of Obstetrics & Gynecology, University of Virginia

Establishing primary endometrial stromal cell culture systems from hysterectomy specimens is a valuable biological technique and a crucial step prior to pursuing a vast array of research aims. Here, we describe two methods used to establish stromal cultures from surgically resected endometrial tissues of human patients.

JoVE Core

Preparation of Aligned Steel Fiber Reinforced Cementitious Composite and Its Flexural Behavior

Ru Mu¹, Luansu Wei¹, Xiaowei Wang¹, Hui Li¹, Longbang Qing¹, Jian Zhou¹, Quanming Zhao²

¹School of Civil and Transportation Engineering, Hebei University of Technology, ²School of Information Engineering, Hebei University of Technology

This protocol describes an approach for manufacturing aligned steel fiber reinforced cementitious composite by applying a uniform electromagnetic field. Aligned steel fiber reinforced cementitious composite exhibits superior mechanical properties to ordinary fiber reinforced concrete.

Bioengineering

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Tatjana Michel¹, Antonia Link¹, Meike-Kristin Abraham^1,2, Christian Schlensak¹, Karlheinz Peter^2,3, Hans-Peter Wendel¹, Xiaowei Wang^2,3, Stefanie Krajewski¹

¹Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, ²Atherothrombosis and Vascular Biology, Baker Heart & Diabetes Institute, ³Department of Medicine, Monash University

Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.

Neuroscience

In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy

Amanda M Sweeney^*1, Virginia Plá^*1, Ting Du¹, Guojun Liu¹, Qian Sun¹, Sisi Peng¹, Benjamin A. Plog¹, Benjamin T. Kress¹, Xiaowei Wang¹, Humberto Mestre¹, Maiken Nedergaard^1,2

¹Center for Translational Neuromedicine, University of Rochester Medical Center, ²Center for Translational Neuromedicine, University of Copenhagen

Transcranial optical imaging allows wide-field imaging of cerebrospinal fluid transport in the cortex of live mice through an intact skull.

Behavior

Using a Virtual Reality Walking Simulator to Investigate Pedestrian Behavior

Hyun Chae Chung¹, Soon Ho Kim², Gyoojae Choi³, Jong Won Kim⁴, Moo Young Choi², Hui Li¹

¹Department of Sport and Exercise Sciences, Kunsan National University, ²Department of Physics and Astronomy, Seoul National University, ³School of Mechanical and Automotive Engineering, Kunsan National University, ⁴Department of Healthcare Information Technology, Inje University

This protocol describes use of a walking simulator that serves as a safe and ecologically valid method to study pedestrian behavior in the presence of moving traffic.

Bioengineering

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering

Sougata Ghosh^1,2,3, Tanay Bhagwat³, Rohini Kitture⁴, Sirikanjana Thongmee¹, Thomas J. Webster^3,5,6,7

¹Department of Physics, Faculty of Science, Kasetsart University, ²Department of Microbiology, School of Science, RK. University, ³Department of Chemical Engineering, Northeastern University, ⁴Defence Institute of Advanced Technology Campus, Navyukti Innovations Pvt. Ltd., ⁵Department of Biomedical Engineering, Hebei University of Technology, ⁶Department of Chemistry, Saveetha School of Engineering, Institute of Medical and Technical Science, Saveetha University, ⁷UFPI - Universidade Federal do Piauí

Novel nanocomposites of graphene nanoribbons and hydroxyapatite nanoparticles were prepared using solution-phase synthesis. These hybrids when employed in bioactive scaffolds can exhibit potential applications in tissue engineering and bone regeneration.

Medicine

Developing and Testing Methylated Nano-Structured Dipeptides that Inhibit Src Kinase Activity In Vitro for Anti-Cancer Applications

Alaa F. Nahhas¹, Alrayan F. Nahhas², Thomas J. Webster^3,4,5

¹Biochemistry Department, College of Science, King Abdulaziz University, ²Independent Scholar, ³School of Health Sciences and Biomedical Engineering, Hebei University of Technology, ⁴School of Engineering, Saveetha University, ⁵Programa de Pós-graduação em Ciência e Engenharia dos Materiais, UFPI

Presented here is a protocol for designing and testing dipeptides that can inhibit Src kinase enzyme activity using acellular and cellular assays for anti-cancer applications. The peptides formulated here (W-R_CH3, W_CH3-R_CH3, and W-R_(CH3)2) inhibited Src kinase with IC₅₀ values of 510 nM, 916 nM, and 1 µM, respectively.

Environment

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique

Siqian Liu¹, Jian Zhou¹, Lingyan Zhu¹

¹College of Natural Resources and Environment, Northwest A&F University

The present protocol describes a simple and efficient method for the long-distance transport of perfluoroalkyl acids in wheat.

Bioengineering

High-Performance Graphene-Modified Sensing Chip for SARS-CoV-2 Detection

Parshant Kumar Sharma^1,2,3, Ebrahim Mostafavi^4,5, Nam-Young Kim^1,2,3, Thomas J. Webster⁶, Ajeet Kaushik^7,8

¹RFIC Bio Centre, Kwangwoon University, ²Department of Electronics Engineering, Kwangwoon University, ³NDAC Centre, Kwangwoon University, ⁴Stanford Cardiovascular Institute, Stanford University School of Medicine, ⁵Department of Medicine, Stanford University School of Medicine, ⁶Department of Biomedical Engineering, Hebei University of Technology, ⁷NanoBioTech Laboratory, Department of Environmental Engineering, Florida Polytechnic University, ⁸School of Engineering, University of Petroleum and Energy Studies (UPES)

The present protocol describes the fabrication of low-cost biosensing prototypes based on useful nanosystems for accurately detecting viral proteins (at the Fg level). Such a tiny sensor platform allows for point-of-care applications that can be integrated with the Internet of Medical Things (IoMT) to meet telemedicine objectives.