Qingdao Agricultural University
2 ARTICLES PUBLISHED IN JoVE
Biology
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
Lina Qiu 1, Daqing Huang 1, Yongzhang Wang 1, Haiyong Qu 1
1College of Horticulture, Qingdao Agricultural University
Isolated protoplasts of apple pulp cells were loaded with a calcium fluorescent reagent to detect cytoplasmic Ca2+ concentration.
Immunology and Infection
Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis
Yan Zhang *1, Xingxing Su *2, Lisha Wang 2, Zhengliang Yue 2, Qiao Liu 3, Ling Ran 2, Shun Lei 2, Jianjun Hu 2, Lifan Xu 2, Lilin Ye 2, Ping Ji 4, Guimei Li 1, Qizhao Huang 5, Shuqiong Wen 4
1College of Veterinary Medicine, Qingdao Agricultural University, 2Institute of Immunology, Third Military Medical University, 3Institute of Cancer, Xinqiao Hospital, Third Military Medical University, 4Stomatological Hospital of Chongqing Medical University, 5Institute of life science, Chongqing Medical University
The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T cells.
