Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevisAlexander Brinkmann *1,2, Camille Okom *1,2, Eugen Kludt 1,2,4, Detlev Schild 1,2,3
1Institute of Neurophysiology and Cellular Biophysics, Georg-August-Universität Göttingen, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Georg-August-Universität Göttingen, 3DFG Excellence Cluster 171, Georg-August-Universität Göttingen, 4German Hearing Center Hannover
Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.
