University of Electronic Science and Technology of China

6 ARTICLES PUBLISHED IN JoVE

Chemistry

Low Pressure Vapor-assisted Solution Process for Tunable Band Gap Pinhole-free Methylammonium Lead Halide Perovskite Films

Carolin M. Sutter-Fella^*1,2,3, Yanbo Li^*1,4, Nicola Cefarin^1,5,6, Aya Buckley^1,7, Quynh Phuong Ngo^8,9, Ali Javey^2,3, Ian D. Sharp¹, Francesca M. Toma¹

¹Joint Center for Artificial Photosynthesis, Chemical Sciences Division, Lawrence Berkeley National Laboratory, ²Electrical Engineering and Computer Sciences, University of California, Berkeley, ³Materials Science Division, Lawrence Berkeley National Laboratory, ⁴Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, ⁵Department of Physics, Graduate School of Nanotechnology, University of Trieste, ⁶TASC Laboratory, IOM-CNR - Istituto Officina dei Materiali, ⁷Department of Chemistry, University of California, Berkeley, ⁸Materials Science and Engineering, University of California, Berkeley, ⁹Joint Center for Artificial Photosynthesis, Lawrence Berkeley National Laboratory

Here, we present a protocol for the synthesis of CH₃NH₃I and CH₃NH₃Br precursors and the subsequent formation of pinhole-free, continuous CH₃NH₃PbI_3-xBr_x thin films for the application in high efficiency solar cells and other optoelectronic devices.

JoVE Core

Generation and Coherent Control of Pulsed Quantum Frequency Combs

Benjamin MacLellan^*1, Piotr Roztocki^*1, Michael Kues^1,2, Christian Reimer¹, Luis Romero Cortés¹, Yanbing Zhang¹, Stefania Sciara^1,3, Benjamin Wetzel^1,4, Alfonso Cino³, Sai T. Chu⁵, Brent E. Little⁶, David J. Moss⁷, Lucia Caspani⁸, José Azaña¹, Roberto Morandotti^1,9,10

¹Institut National de la Recherche Scientifique - Centre Énergie, Matériaux et Télécommunications (INRS-EMT), ²School of Engineering, University of Glasgow, ³Department of Energy, Information Engineering and Mathematical Models, University of Palermo, ⁴School of Mathematical and Physical Sciences, University of Sussex, ⁵Department of Physics and Material Science, City University of Hong Kong, ⁶State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Science, ⁷Centre for Micro Photonics, Swinburne University of Technology, ⁸Institute of Photonics, Department of Physics, University of Strathclyde, ⁹Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, ¹⁰National Research University of Information Technologies, Mechanics and Optics

A protocol is presented for the practical generation and coherent manipulation of high-dimensional frequency-bin entangled photon states using integrated micro-cavities and standard telecommunications components, respectively.

Immunology and Infection

Enhancement Method of Surface Acoustic Wave-Atomizer Efficiency for Olfactory Display

Takamichi Nakamoto¹, Sami Ollila¹, Shingo Kato¹, Haining Li^1,2, Guiping Qi¹

¹Tokyo Institute of Technology, ²University of Electronic Science and Technology of China

We establish here a method for coating the surface of a surface acoustic wave (SAW) device with amorphous Teflon film to improve the atomization efficiency required for application to an olfactory display.

Developmental Biology

Monocular Visual Deprivation and Ocular Dominance Plasticity Measurement in the Mouse Primary Visual Cortex

Ke Chen^*1,2, Yilei Zhao^*1, Ting Liu¹, Zhaohao Su¹, Huiliang Yu¹, Leanne Lai Hang Chan^3,4, Tiejun Liu^1,2, Dezhong Yao^1,2

¹The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for NeuroInformation, University of Electronic Science and Technology of China, ²Sichuan Institute for Brain Science and Brain-inspired Intelligence, ³Department of Electronic Engineering, City University of Hong Kong, ⁴Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong

Here, we present detailed protocols for monocular visual deprivation and ocular dominance plasticity analysis, which are important methods for studying the neural mechanisms of visual plasticity during the critical period and the effects of specific genes on visual development.

Biology

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay

Yuping Fan^*1,2, Yan Teng^*3, Fu tong Liu^*4, Fengxia Ma^1,2, Alan Y. Hsu⁵, Sizhou Feng^1,2, Hongbo R. Luo⁵

¹State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, ²Tianjin Institutes of Health Science, ³Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, ⁴Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, ⁵Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center

This protocol details the preparation of CLON-G to extend the neutrophil lifespan to greater than 5 days and provides a reliable procedure for evaluating neutrophil death with flow cytometry and confocal fluorescence microscopy.

Neuroscience

Whole-Mount Immunostaining and Automatic Counting of Mouse Retinal Ganglion Cells

Jialiang Yang^*1, Jiaxin Guo^*1, Jing Hu^*1,2, Xiawei Wu^*1, Haotian Huang¹, Yu Wen¹, Runfang Chen¹, Congrui Liu¹, Houbin Zhang^1,3

¹Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, ²School of Medicine, University of Electronic Science and Technology of China, ³Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

This protocol describes the procedure for isolating the whole-mount mouse retina and performing immunostaining to label all retinal ganglion cells (RGCs). The process is followed by imaging and automatically counting RGCs using AI-based software, providing a simple, fast, and accurate method for quantifying RGCs in the entire mouse retina.