An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal MicrobiotaShokouh Ahmadi *1,2,3, Shaohua Wang *1,2, Ravinder Nagpal *1,2, Rabina Mainali 1,2, Sabihe Soleimanian-Zad 3,4, Dalane Kitzman 5, Hariom Yadav 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
This protocol describes an in vitro batch-culture fermentation system of human fecal microbiota, using inulin (a well-known prebiotic and one of the most widely studied microbiota modulators) to demonstrate the use of this system in estimating effects of specific interventions on fecal microbiota composition and metabolic activities.
