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Doshisha Women's College

1 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System
Takayuki Ueno 1,2, Kiyoshi Kawasaki 2, Takeo Kubo 1
1Department of Biological Sciences, Graduate School of Sciences, The University of Tokyo, 2Faculty of Pharmaceutical Sciences, Doshisha Women's College

Here we describe our detailed protocol for the preparation of single-cohort honeybee colonies – a useful tool for analyzing the role-associated worker physiology. We also describe detailed protocols for treating workers with juvenile hormone and ecdysone to evaluate the involvement of these hormones in the regulation of worker behavior and/or physiology.

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