Zhejiang agricultural & forestry University

2 ARTICLES PUBLISHED IN JoVE

Biology

Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus

Najie Zhu^*1, Liqun Bai^*1, Stefan Schütz², Baojun Liu³, Zhenyu Liu³, Xingyao Zhang⁴, Hongshi Yu¹, Jiafu Hu^1,5

¹Department of Forest Protection, Zhejiang Agricultural & Forestry University, ²Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, ³College of Plant Protection, Shandong Agricultural University, ⁴Institute of Forest Protection, Chinese Academy of Forestry, ⁵Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University

A protocol for investigating the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, is presented. Behavioral features of B. xylophilus are described in the mating process.

Biology

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Xiaofang Liu¹, Xiang Zhou¹, Lifeng Zhou¹, Jiafu Hu¹, Kai Guo¹

¹College of Forestry and Biotechnology, Zhejiang Agricultural & Forestry University

Here, we introduce a detailed soaking method of RNA interference in Bursaphelenchus xylophilus to facilitate the study of gene functions.