A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic TreatmentBradley Endres 1, Eugénie Bassères 1, Tasnuva Rashid 1, Long Chang 2, M. Jahangir Alam 1, Kevin W Garey 1
1Department of Pharmacy Practice and Translational Research, University of Houston College of Pharmacy, 2Department of Electrical and Computer Engineering, University of Houston
Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.
