Radboudumc

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Mariateresa Tedesco¹, Monica Frega^1,2, Sergio Martinoia¹, Mattia Pesce³, Paolo Massobrio¹

¹Department of Informatics, Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, ²Donders Institute for Brain, Cognition and Behaviour, Department of Cognitive Neuroscience, Radboud University Medical Center, ³Fondazione Istituto Italiano di Tecnologia (IIT)

In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.

Developmental Biology

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Monica Frega^*1,2, Sebastianus H. C. van Gestel^*3, Katrin Linda^2,3, Jori van der Raadt³, Jason Keller^1,2, Jon-Ruben Van Rhijn^1,2, Dirk Schubert^1,2, Cornelis A. Albers^2,3,4, Nael Nadif Kasri^1,2,3

¹Department of Cognitive Neurosciences, Radboudumc, ²Donders Institute for Brain, Cognition and Behaviour, Radboud University, ³Department of Human Genetics, Radboudumc, ⁴Department of Molecular Developmental Biology, Radboud University

We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons¹². Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.

Genetics

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

Sapthami Nadesapillai¹, Janielle van der Velden², Didi Braat¹, Kathrin Fleischer^1,3, Ronald Peek¹

¹Department of Obstetrics and Gynecology, Radboudumc, ²Department of Pediatrics, Radboudumc, Amalia Children’s Hospital, ³Department of Reproductive Medicine, Nij Geertgen Center for Fertility

This article presents two methods based on fluorescence in situ hybridization to determine the X chromosomal content of ovarian cells in non-grafted and grafted ovarian cortex tissue from females with X chromosomal aberrations.

Cancer Research

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Mark A. J. Gorris^1,2, Evgenia Martynova^*1,3, Mark W. D. Sweep^*1,4, Iris A. E. van der Hoorn^1,5, Shabaz Sultan^1,3, Mike J. D. E. Claassens¹, Lieke L. van der Woude^1,2,6, Kiek Verrijp^1,2,6, Carl G. Figdor^1,3, Johannes Textor^1,3, I. Jolanda M. de Vries¹

¹Department of Medical BioSciences, Radboudumc, ²Division of Immunotherapy, Oncode Institute, Radboudumc, ³Data Science, Institute for Computing and Information Sciences, Radboud University, ⁴Department of Medical Oncology, Radboudumc, ⁵Department of Pulmonary Diseases, Radboudumc, ⁶Department of Pathology, Radboudumc

This protocol describes in detail how immune cell characterization of the tumor microenvironment using multiplex immunohistochemistry is carried out.