Hunan University

3 ARTICLES PUBLISHED IN JoVE

Education

The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy

Libo Zhang¹, Junjie Zhu², Hao Ren², Dongdong Liu³, Dan Meng⁴, Yanjun Wu¹, Tiejian Luo⁵

¹Institute of Software, Chinese Academy of Sciences, ²Hunan University, ³Fuyang Normal University, ⁴Anhui Normal University, ⁵University of Chinese Academy of Sciences

We present a protocol on modular design and production of intelligent robots to help scientific and technical workers design intelligent robots with special production tasks based on personal needs and individualized design.

Biology

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Jing Zhang^*1, Jing Huang^*2, Ishani Majumdar¹, Ryan C. James³, Julia Gichimu¹, Manikandan Paramasivam⁴, Durga Pokharel⁵, Himabindu Gali⁶, Marina A. Bellani¹, Michael M Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, ²Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, ³Department of Molecular Biology and Genetics, Cornell University, ⁴Department of Cellular and Molecular Medicine, University of Copenhagen, ⁵Horizon Discovery, ⁶Boston University School of Medicine

While replication fork collisions with DNA adducts can induce double strand breaks, less is known about the interaction between replisomes and blocking lesions. We have employed the proximity ligation assay to visualize these encounters and to characterize the consequences for replisome composition.

Genetics

Comparative Karyotype Analysis of Lycoris aurea Herb Populations Using Fluorochrome Banding and 45S and 5S rDNA-FISH

Xianghui Jiang¹, Miaohua Quan¹, Chaowen She²

¹Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, ²College of Biological and Food Engineering, Hunan University

This protocol investigates the heterochromatin distribution in eight populations of Lycoris aurea (L′Her) Herb, using combined PI and DAPI chromosome staining, fluorescence in situ hybridization (FISH) with 18S-5.8-26S rRNA gene regions (45S rDNA regions), and 5S ribosomal DNA probes.