Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter SystemsYu-Xiang Chen 1, Miamiao Pan 1, Yue-Meng Chen 1, Babak Javid 1
1Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, Tsinghua University School of Medicine
In this article, we present two complementary methods to measure specific rates of translational error and mistranslation in the model mycobacterium, Mycobacterium smegmatis, using gain-of-function reporter systems. The methods can be employed to measure accurate error rates in low throughput or relative error rates in a more high-throughput setting.
