CHA University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Dongyoon Shin^1,2, Dayeon Lee¹, Young June Jeon¹, Kyoungbun Lee³, Youngsoo Kim², Junho Park^1,2

¹Department of Medical Science, School of Medicine, CHA University, ²Department of Proteomic Research, CHA Future Medicine Research Institute, ³Department of Pathology, Seoul National University Hospital

Here, we establish a mass spectrometry-based proteomic method using isolated regions of interest in formalin-fixed, paraffin-embedded tissue sections. This protocol is used to analyze proteome from specific tissue areas in archived formalin-fixed, paraffin-embedded tissue sections.