Automated Robotic Liquid Handling Assembly of Modular DNA DevicesLuis Ortiz 1,2, Marilene Pavan 2, Lloyd McCarthy 3, Joshua Timmons 3, Douglas M. Densmore 4
1Graduate Program in Molecular Biology, Cell Biology, and Biochemistry, Boston University, 2Biological Design Center, Boston University, 3Lattice Automation, 4Department of Electrical and Computer Engineering, Biological Design Center, Boston University
Here, an automated workflow to perform modular DNA "device" assembly using a modular cloning DNA assembly method on liquid-handling robots is presented. The protocol uses an intuitive software tool for generating liquid handler picklists for combinatorial DNA device library generation, which we demonstrate using two liquid handling platforms.
