Children's National Health System
2 ARTICLES PUBLISHED IN JoVE
Cancer Research
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
Tsung-Cheng Lin 1, Ying-Chih Liao 2, Wen-Tsan Chang 1,2, Cheng-Han Yang 1, Li-Hsin Cheng 1, Megan Cheng 3, Hung-Chi Cheng 1,2
1The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 3Trauma Office, Children's National Health System
An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.
Cancer Research
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
Erin R. Bonner 1,2, Karim Saoud 1, Sulgi Lee 1,2, Eshini Panditharatna 3, Madhuri Kambhampati 1, Sabine Mueller 4,5, Javad Nazarian 1,2,5
1Center for Genetic Medicine, Children's National Health System, 2Institute for Biomedical Sciences, The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology, University of California San Francisco, 5DIPG Centre of Expertise Zurich, Universitats-Kinderspital Zurich
Here, we present a protocol to detect tumor somatic mutations in circulating DNA present in patient biological fluids (biofluids). Our droplet digital polymerase chain reaction (dPCR)-based method enables quantification of the tumor mutation allelic frequency (MAF), facilitating a minimally invasive complement to diagnosis and temporal monitoring of tumor response.
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