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Yamanashi Central Hospital

1 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
Kenji Amemiya 1,2, Yosuke Hirotsu 1, Toshio Oyama 2, Masao Omata 1,3
1Genome Analysis Center, Yamanashi Central Hospital, 2Pathology Division, Laboratory Department, Yamanashi Central Hospital, 3The University of Tokyo

Obtaining high-quality genomic DNA from tumor tissues is an essential first step for analyzing genetic alterations using next generation sequencing. In this article, we present a simple and rapid method to enrich tumor cells and obtain intact DNA from touch imprint cytology specimens.

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