Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9Amanda Haupt 1, Tanya Grancharova 1, Joy Arakaki 1, Margaret A. Fuqua 1, Brock Roberts 1, Ruwanthi N. Gunawardane 1
1Allen Institute for Cell Science
Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.
