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National High Magnetic Field Laboratory

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Alex Kirui *1, Malitha C. Dickwella Widanage *1, Frederic Mentink-Vigier 2, Ping Wang 3, Xue Kang 1, Tuo Wang 1
1Department of Chemistry, Louisiana State University, 2National High Magnetic Field Laboratory, 3Departments of Pediatrics, and Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center

A protocol for preparing 13C,15N-labeled fungal and plant samples for multidimensional solid-state NMR spectroscopy and dynamic nuclear polarization (DNP) investigations is presented.

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