Njala University

1 ARTICLES PUBLISHED IN JoVE

Genetics

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Andreas Mueller¹, Kerstin Fischer², Roland Suluku³, Thomas Hoenen¹

¹Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, ²Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, ³Department of Animal Science, Njala University

Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such conditions.