New York Blood Center
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
Lanying Du 1, Xiujuan Zhang 1, Jixiang Liu 1, Shibo Jiang 1
1Lindsley F. Kimball Research Institute, New York Blood Center
This protocol describes a general procedure for studying recombinant receptor-binding domain (RBD)-based subunit vaccines against SARS. It includes methods for transfection and expression of RBD protein in 293T cells, immunization of mice with RBD and detection of neutralization activity of mouse sera using an established SARS pseudovirus neutralization assay.
Immunology and Infection
Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
Nermi L. Parrow *1, Pierre-Christian Violet *2, Hongbin Tu 2, James Nichols 3, Corinne A. Pittman 4, Courtney Fitzhugh 4, Robert E. Fleming 1,5, Narla Mohandas 6, John F. Tisdale 3, Mark Levine 2
1Department of Pediatrics, Saint Louis University School of Medicine, 2Molecular and Clinical Nutrition Section, Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, 3Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, 4Sickle Cell Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 5Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 6Red Cell Physiology Laboratory, New York Blood Center
Here we present techniques to measure red cell deformability and cellular heterogeneity by ektacytometry. These techniques are applicable to general investigations of red cell deformability and specific investigations of blood diseases characterized by the presence of both rigid and deformable red cells in circulation, such as sickle cell anemia.
