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Kaplan Medical Center

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Engineered Vascularized Muscle Flap
Dana Egozi *1, Yulia Shandalov *2, Alina Freiman 2,3, Dekel Rosenfeld 2, David Ben-Shimol 4, Shulamit Levenberg 2
1Department of Plastic Surgery, Kaplan Medical Center, 2Biomedical Engineering, Technion Israel Institute of Technology, 3Interdepartmental Program in Biotechnology, Technion Israel Institute of Technology, 4Medicine Department, Technion Israel Institute of Technology

To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.

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Cancer Research

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
Dimitri Gaber 1, Michal Aharoni-Simon 1, Ortal Zaks 1, Keren Ben-Yaakov 1, Ziv Rotfogel 1,2, Hana Leiba 1,2, Avital Eisenberg-Lerner *1, Arie L. Marcovich *1,2
1Ophthalmology Research Laboratory, Kaplan Medical Center, 2Department of Ophthalmology, Kaplan Medical Center, Faculty of Medicine, The Hebrew University of Jerusalem

The present protocol describes the implantation and evaluation of melanoma in the murine choroid utilizing optical coherence tomography.

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Biology

Intracameral Injection in Rats with Low Risk of Adverse Effects
Oriel Ratzon *1, Mor Schlesinger *1, Keren Ben-Yaakov 1, Ortal Zaks 1, Ziv Rotfogel 1,2, Arie L. Marcovich 1,2, Avital Eisenberg-Lerner 1
1Ophthalmology Research Laboratory, Kaplan Medical Center, 2Department of Ophthalmology, Kaplan Medical Center, Faculty of Medicine, Hebrew University of Jerusalem

This protocol describes a technique for intracameral injection in rats using a central corneal incision and a long tunnel into the anterior chamber. This injection method minimizes the risk of inducing inadvertent tissue damage and thereby improves precision and reproducibility.

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