SRI International

2 ARTICLES PUBLISHED IN JoVE

Medicine

Gene-environment Interaction Models to Unmask Susceptibility Mechanisms in Parkinson's Disease

Vivian P. Chou¹, Novie Ko¹, Theodore R. Holman², Amy B. Manning-Boğ¹

¹Center for Health Sciences, SRI International, ²Department of Chemistry and Biochemistry, University of California-Santa Cruz

Lipoxygenase (LOX) isozymes can generate products that may increase or decrease neuroinflammation and neurodegeneration. A gene-environment interaction study could identify LOX isozyme-specific effects. Using the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of nigrostriatal damage in two LOX isozyme-deficient transgenic lines allows for comparison of the contribution of LOX isozymes on dopaminergic integrity and inflammation.

Cancer Research

Capture and Release of Viable Circulating Tumor Cells from Blood

Siddarth Rawal¹, Zheng Ao², Ashutosh Agarwal^1,3

¹Department of Pathology, University of Miami, ²SRI International, ³Department of Biomedical Engineering, DJTMF Biomedical Nanotechnology Institute at the University of Miami, University of Miami

A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.