AstraZeneca

4 ARTICLES PUBLISHED IN JoVE

Chemistry

A New Straightforward Method for Lipophilicity (logP) Measurement using ¹⁹F NMR Spectroscopy

Zhong Wang¹, Benjamin F. Jeffries¹, Hannah R. Felstead¹, Neil J. Wells¹, Elisabetta Chiarparin², Bruno Linclau¹

¹Chemistry, University of Southampton, ²Medicinal Chemistry, Oncology, IMED Biotech Unit, AstraZeneca

A novel and straightforward variation of the shake-flask method was developed for accurate lipophilicity measurement of fluorinated compounds by ¹⁹F NMR spectroscopy.

Biology

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)

Ida Alexandersson¹, Matthew J. Harms¹, Jeremie Boucher^1,2,3

¹Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, ²The Lundberg Laboratory for Diabetes Research, University of Gothenburg, ³Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg

Membrane mature adipocyte aggregate culture (MAAC) is a new method to culture mature human adipocytes. Here we detail how to isolate adipocytes from human adipose and how to set up MAAC.

Medicine

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Paola Marco-Casanova¹, Natalia Lukashchuk¹, Benedetta Lombardi¹, Veerendra Munugalavadla², Melanie M. Frigault³, Elizabeth A. Harrington¹, J. Carl Barrett³, Andrew J. Pierce¹

¹Translational Medicine, R&D Oncology, AstraZeneca, ²Acerta Pharma, AstraZeneca Group, ³Translational Medicine, R&D Oncology, AstraZeneca

This protocol details clinically implementable preparation of high quality PBMC and plasma biosamples at the clinical trial site that can be used for translational biomarker analysis.

Genetics

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

Payam Haftbaradaran Esfahani¹, Ralph Knöll^1,2

¹Department of Medicine, Integrated Cardio Metabolic Centre (ICMC), Heart and Vascular Theme, Karolinska Institutet, ²Bioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca

This paper presents methods for growing cardiac myocytes with different shapes, which represent different pathologies, and sorting these adherent cardiac myocytes based on their morphology at a single cell level. The proposed platform provides a novel approach to high throughput and drug screening for different types of heart failure.