Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In SituIsabella Cattani-Cavalieri 1, Jordyn Margolis 1, Camelia Anicolaesei 1, Francisco J. Nuñez 1, Rennolds S. Ostrom 1
1Department of Biomedical and Pharmaceutical Sciences, Chapman University School of Pharmacy
The protocol presented in this study illustrates the effectiveness of the cAMP Difference Detector In Situ in measuring cAMP through two methods. One method utilizes a 96-well plate reading spectrophotometer with HEK-293 cells. The other method demonstrates individual HASM cells under a fluorescent microscope.
