Amsterdam Cardiovascular Sciences

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Aida Llucià-Valldeperas^1,2, Ramon Bragós³, Antoni Bayés-Genís^1,4,5,6

¹Insuficiencia Cardiaca y Regeneración Cardiaca (ICREC) Research Program, Health Science Research Institute Germans Trias i Pujol, ²Amsterdam Universitair Medisch Centrum (UMC), Vrije Universiteit Amsterdam, Pulmonology and Physiology, Amsterdam Cardiovascular Sciences, ³Electronic and Biomedical Instrumentation Group, Departament d'Enginyeria Electrònica, Universitat Politècnica de Catalunya, ⁴Cardiology Service, Germans Trias i Pujol University Hospital, ⁵Department of Medicine, Universitat Autònoma de Barcelona, ⁶Centro de Investigación Biomédica en Red (CIBER) Cardiovascular, Instituto de Salud Carlos III

Here we present a protocol for training a cell population using electrical and mechanical stimuli emulating cardiac physiology. This electromechanical stimulation enhances the cardiomyogenic potential of the treated cells and is a promising strategy for further cell therapy, disease modeling, and drug screening.

Biology

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Rafeeh Dinani^1,2, Emmy Manders^1,2,3, Michiel Helmes^1,2,3, Lili Wang⁴, Bjorn C. Knollmann⁴, Diederik W. D. Kuster^1,2, Jolanda van der Velden^1,2

¹Division of Physiology, Amsterdam UMC, Vrije University, ²Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology, Vanderbilt School of Medicine

Here, an established method is described to perform contractility and calcium measurements in human induced pluripotent stem cell-derived cardiomyocytes using an optics-based platform. This platform enables researchers to study the effect of mutations and the response to various stimuli in a rapid and reproducible manner.